Figure 7. IL6/STAT3 axis activation increased miR-483-3p expression. (A, D) qRT-PCR analysis showed the expression level of miR-483-3p in U251 and U87 cells after treatment of STAT3 phosphorylation inhibitor. Data are mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. (B, E) Western bolt analysis showed protein levels of p-STAT3 and total STAT3 in U251 and U87 cells. Data are mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. (C, F) qRT-PCR analysis showed the expression level of miR-483-3p in U251 and U87 cells after IL-6 overexpression and neutralization and STAT3 inhibition. Data are mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. **P<0.05.