Figure 8. miR-483-3p promoted in vivo tumor growth in the nude mice. (A) Tumor volume was increased after miR-483-3p overexpression. Data are mean ± SEM; Two-tailed t test was used for the statistical analysis. n=5 mice. (B) Tumor weight was increased after miR-483-3p overexpression. Data are mean ± SEM; Two-tailed t test was used for the statistical analysis. n=5 mice. (C) RT-PCR showed miR-483-3p level. Data are mean ± SEM; Two-tailed t test was used for the statistical analysis. n=6 mice. (D) RT-PCR showed mRNA level of EMT related markers. Data are mean ± SEM; Two-tailed t test was used for the statistical analysis. n=5 mice. (E) Western blot showed protein level of EMT markers. Data are mean ± SEM; Two-tailed t test was used for the statistical analysis. n=5 mice. **P<0.05. (F) Pulmonary metastasis of glioma was detected. n=5 mice.