Research Paper Volume 12, Issue 19 pp 19335—19351

FREM1 expression is reduced in tumor tissues, which is associated with poor survival. (A) Representative IHC staining of FREM1 in human primary tumor and adjacent non-tumor tissue. Magnifications: 4X; boxed area is 40X. Scale bar: 4X, 200 μm; 40X, 20 μm. (B) FREM1 level is higher in adjacent tissues than in tumor tissues. Magnifications: 4X; and boxed area is 40X. Scale bar: 4X, 200 μm; 40X, 20 μm. (C, D) Analysis of FREM1 IHC staining scores in adjacent non-tumor (n = 48) and tumor tissue (n = 56). (*P < 0.05, **P < 0.01, ***P < 0.001). (E–H) Survival curves of OS and DFS in breast cancer and HER2 with low and high FREM1 expression. Median survival time of the high-expression group versus low-expression group.