Research Paper Volume 12, Issue 18 pp 18588—18602

Lipopolysaccharide upregulates miR-132/212 in Hirschsprung-associated enterocolitis, facilitating pyroptosis by activating NLRP3 inflammasome via targeting Sirtuin 1 (SIRT1)


Figure 3. SIRT1 is a direct target gene of miR-132 and 212. (A and B) qRT-PCR and western blot analysis validated respectively mRNA and protein levels of SIRT1 after transfection with miR-132/212 mimics (or Con mimics) in HT29 cell line. Mean ± SD. n = 3, *P < 0.05. (C) The binding sites within wild type or mutant SIRT1 3’UTR and miR-132 and miR-212. The luciferase activity of wild type or mutant SIRT1 3’UTR after transfection with miR-132 or miR-212 mimics was assessed by dual luciferase reporter assay in HT29 cell line. Con, control.