Research Paper Volume 13, Issue 9 pp 13225—13238

TRIM14 regulates melanoma malignancy via PTEN/PI3K/AKT and STAT3 pathways


Figure 7. Overexpression of TRIM14 promoted melanoma cell proliferation via AKT and STAT3 pathways. A375 cells were transfected with Oe-NC and Oe-TRIM14, and the protein expression of TRIM14 after transfection was determined using western blot (A). A375 cells were transfected with Oe-TRIM14 with or without the treatment of LY294002 (an AKT pathway inhibitor) or SH-4-54 (a STAT3 pathway inhibitor). After transfection, cell proliferation ability was determined using CCK-8 assay (B) and clone formation assay (C). **p < 0.01 vs Oe-NC; ###p < 0.001 vs Oe-TRIM14.