Research Paper Volume 13, Issue 16 pp 20651—20660

NLRC5 attenuates inflammatory response in IL-1β-stimulated human osteoarthritis chondrocytes through the NF-κB signaling pathway


Figure 7. Inhibition of NF-κB partially reversed the si-NLRC5-mediated promotion of IL-1β-induced inflammatory injury in chondrocytes. Chondrocytes were treated with PDTC (5 ;μM) to prevent the activation of NF-κB signaling pathway. (A) Cell viability of chondrocytes was detected using MTT assay. (B, C) The mRNA levels of iNOS and COX-2 were measured using RT-PCR. (DG) The production of TNF-α, IL-6, MMP-3/13 in chondrocytes. *p < 0.05.