Research Paper Volume 14, Issue 22 pp 9243—9263
System analysis based on the ER stress-related genes identifies WFS1 as a novel therapy target for colon cancer
Figure 4. Knockdown of WFS1 inhibits COAD cell proliferation. (A) Box plots showed that WFS1 expression was significantly higher in COAD than in normal samples. (B) Survival curves showed that patients in the low WFS1 expression group had a better prognosis than those in the high WFS1 expression group. (C) Western blot analysis of WFS1 expression in HCT116 and DLD-1 cell lines. actin is internal control. (D) Cell viability of HCT116 or DLD-1 cells transfected with control or WFS1siRNAs was measured by CCK8 assay. (E) Clone formation assay of HCT116 or DLD-1 cells transfected with control or WFS1siRNAs. (F) EdU staining for cell proliferation transfected with control or WFS1siRNAs.