Research Paper Volume 15, Issue 5 pp 1591—1602

Figure 1. Aβ and P-tau expression in mice. Two weeks after stereotaxic injection, the expression of Aβ and P-tau in the hippocampus of the three groups of mice were detected by Western blot (A). The gray value analysis showed that there was no significant difference in the expression of Aβ between the normal group and the control group, and were significantly lower than the okadaic acid (OA) group (B) (ns: P = 0.985; ^***P < 0.001) (n = 6, LSD test). The expression of PP2A of OA group was obviously lower than that of the normal group and the control group (C), but there is no difference between the normal and control group (ns: P = 0.792; ^***P < 0.001). Compared with the normal group and the control group, the PP2A-Yp307 (D) (^***P < 0.001), P-tau (Ser396/404) (E) (^***P < 0.001) and P-tau (Thr 231) (F) (^***P < 0.001) of OA group were significantly higher, but there was no significant difference between the normal group and the control group and the P values were 0.582, 0.936 and 0.600 respectively. The expression of tau-5 (G) was not significantly different among the 3 groups (ns, P = 0.724 and 0.799). Normal: Mice without stereotactic puncture; control: The mice were punctured stereotaxically and injected with 5 μL of normal saline; OA: Mice were subjected to stereotactic puncture and injection of 5 μL of 0.1 μM okadaic acid. (LSD test, n = 6).