Research Paper Volume 15, Issue 6 pp 1808—1832
Cellular senescence and disrupted proteostasis induced by myotube atrophy are prevented with low-dose metformin and leucine cocktail
Figure 3. Low concentration MET+LEU uniquely prevents myotube atrophy and alters transcriptional profile during SD. (A–C) Representative images of 2% HS, SD, and 0.1 + 0.5 μM MET+LEU. (D) Myotube area observing the effects of low concentration MET, LEU, and MET+LEU after 4 days of SD as a fold change from 2% HS. (E) The percent change in upregulated and downregulated transcripts in SD vs. 2% HS, MET vs. SD, LEU vs. SD, and MET+LEU vs. SD with the number of transcripts changed above each bar. (F) Principle component analysis based on the top 500 transcripts changed with 2% HS, SD, and low concentration MET, LEU, and MET+LEU. *p < 0.05. N = 9/group for (A–D), N = 6/group for (E and F). Scale bar represents 200 μm.