Research Paper Volume 15, Issue 24 pp 14764—14790

Conjoint research of WGCNA, single-cell transcriptome and structural biology reveals the potential targets of IDD development and treatment and JAK3 involvement

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Figure 3. (A) tSNE visualization of all cells in NP tissues after sample integration, 9 clustered were generated. (B) Dot plot showing the NP cells marker genes ACAN, SOX9 and MIA within tSNE map; and violin plot showing the expression of macrophage marker genes CD14 and MRC1. (C) tSNE visualization of all cells in NP tissues between degeneration and non-degeneration group. (D) Selection of the most variable genes for monocle analysis. (E) PCA dimensional reduction by monocle algorithm between degeneration and non-degeneration group. (F) Volcano scatter plot of the genes analyzed by monocle method. (G) Hierarchical clustering heatmap of the analyzed DEGs in each cell.