ANGPTL4, a direct target of hsa-miR-133a-3p, accelerates lung adenocarcinoma lipid metabolism, proliferation and invasion

Qihao Hu; Shi Chen; Yukun Li; Teng Hu; Jianpeng Hu; Cheng Wang; Fei Yang; Xiang Yang; Feng Zhou; Zhengdong Liu; Wei Xu; Ji Zhang

doi:doi:10.18632/aging.205313

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Research Paper Advance Articles

ANGPTL4, a direct target of hsa-miR-133a-3p, accelerates lung adenocarcinoma lipid metabolism, proliferation and invasion

Figure 2. The effect of ANGPTL4 on LUAD cell proliferation, lipids production, migration and invasion. (A) The expression of ANGPTL4 and FASN in ANGPTL4 KD A549 and H1975 cell by WB and PCR. (B) The effect of ANGPTL4 KD on lipids metabolism by oil red O. MTT analysis (C), Clone formation assay (D), and EdU staining (E) were performed to confirm the effect of ANGPTL4 KD on cell proliferation. Wound healing assay (F) and Transwell invasion assay (G) were performed to confirm the effect of ANGPTL4 KD on cell migration and invasion. ^**P < 0.01, ^***P < 0.001 indicates statistical significance compared with the control.