Research Paper Volume 16, Issue 6 pp 5184—5206

Figure 4. Inhibition of autophagy reverses the effects of BMSCs-derived exosomes on pyroptosis and functional recovery after SCI. (A) Representative immunostaining image of IBA1 and p62 in mice on the 7th day after injury; (B) Quantification of fluorescence intensity; (C) Representative immunostaining image of IBA1 and Beclin1 in mice on the 7th day after injury; (D) Quantification of fluorescence intensity; (E) Representative immunostaining image of IBA1 and Caspase-1 in mice on the 7th day after injury; (F) Quantification of fluorescence intensity; (G) Representative immunostaining image of IBA1 and GSDMD in mice on the 7th day after injury; (H) Quantification of fluorescence intensity; (I) BMS was used to functionally grade mice in different groups on the 28th day after injury; (J) Footprint analysis demonstrated that 3MA reverses the effects of BMSCs-derived exosomes; (K) The footprint quantification of mice walking after SCI (n = 6); (L) The swimming test at Day 28 postinjury; (M) the Louisville Swim Scale score at Day 28 postinjury (n = 6); (N) The MEP analysis was used as electrophysiological assessment after SCI; (O) Quantification of MEP amplitudes and latencies in mice (n = 6) (^*p < 0.05, ^**p < 0.01,^***p < 0.001).