Antagomir of miR-31-5p modulates macrophage polarization via the AMPK/SIRT1/NLRP3 signaling pathway to protect against DSS-induced colitis in mice

Yuyi Yuan; Shuangjiao Deng; Jia Yang; Zhexing Shou; Chunzhu Wei; Lijuan Zhang; Feng Zhu; Fei Gao; Xingxing Liu; Yujin Liu; Qianyun Chen; Heng Fan

doi:doi:10.18632/aging.205651

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Research Paper Volume 16, Issue 6 pp 5336—5353

Antagomir of miR-31-5p modulates macrophage polarization via the AMPK/SIRT1/NLRP3 signaling pathway to protect against DSS-induced colitis in mice

Figure 8. miR-31-5p antagomir may transform macrophages from M1 type to M2 type through the AMPK-related pathway. (A) miR-31-5p antagomir was successfully transfected into RAW264.7 cells. (B) qPCR detection of AMPK, NOS2, and Arg1 mRNAs. (C) Western blotting strips of P-AMPK/AMPK, NOS2, Arg1, NOX2, HADHB (β-actin). (D) Protein quantitative analysis of P-AMPK/AMPK, NOS2, Arg1, NOX2 and HADHB (β-actin). (E) Flow cytometry of M1/M2. (F) Statistical analysis of M1/M2 cell ratio. Each bar represents mean ± SD, n = 8 from each group. Ns represented P > 0.05, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001 vs. LPS group.