Research Paper Volume 16, Issue 6 pp 5567—5580

Figure 8. CRIF1 overexpression alleviated oxidative stress and cell senescence by inhibiting PXDN in DOX-induced AC16 cells. (A) AC16 cells were transfected with Ov-NC or Ov-PXDN, and the overexpression efficacy of PXDN was detected by western blot. ***P<0.001 vs Ov-NC. (B) DCFH-DA staining was used to detect ROS levels. (C) The expression levels of MDA, GSH and CAT in cells were detected by biochemical kits. (D) SA-β-gal staining was used to observe the level of cell senescence. (E, F) RT-qPCR and western blot detected the expression of p27, p21, and p16. ***P<0.001 vs Control; ###P<0.001 vs DOX; +P<0.05, ++P<0.01, +++P<0.001 vs DOX+Ov-CRIF1+Ov-NC.