Research Paper Volume 16, Issue 6 pp 5601—5617
WTAP regulates the production of reactive oxygen species, promotes malignant progression, and is closely related to the tumor microenvironment in glioblastoma
Figure 5. WTAP promotes ROS production. (A, B) RT-PCR (A) and western blotting (B) were used to analyze the differential expression of WTAP among NHA, U87, U118, LN229, and U251 cell lines. (C, D) The knockdown efficiency of shWTAP-1, -2, and -3 in U251 cells was analyzed by RT-PCR (C) and western blotting (D). shWTAP-1 was chosen in subsequent experiments because of its outstanding efficiency in silencing WTAP. (E, F) The overexpression efficiency of WTAP in U87 cells transfected with lentivirus was analyzed by RT-PCR (E) and western blotting (F). (G, H) The effect of the change of WTAP expression level on ROS production in U87 (G) and U251 (H) cells was detected by a fluorescent microplate reader. (I, J) WTAP participates in the regulation of CAT and SOD1 protease expression in U87 (I) and U251 (J) cells. *P < 0.05; **P < 0.01; ***P < 0.001.