Research Paper Volume 16, Issue 6 pp 4980—4999

Figure 5. Liver protein markers were evaluated in mice fed with CMS121 for 6 months. Representative blot images (A), and the corresponding quantification (B–R) of metabolism markers in the hepatic tissue: FASN (B), PEPCK (C), p-LDH-A (D), PFKFB3 (E), TXNIP (F), MLYCD (G), malonylation of proteins at lysine residues (MAL-K, (H)), FH (I), Nox4 (J), caspase 1 (K), caspase 3 (L), and markers of mitochondrial complex I ((M); NDUFB8), complex II ((N); SDHB), complex III ((O); UQCRC2), and complex V ((P); ATP5A), as well as the voltage dependent anion channel VDAC (Q), and the outer membrane translocase TOM20 (R). Liver cytosolic fractions were used for blotting, except for the mitochondrial markers (M–R), FH (I), and NOX4 (J) that used mitochondrial fractions. Data are presented as mean ± SD (N = 6–8). Bold underlined p-values indicate statistical differences.