Research Paper Volume 16, Issue 6 pp 5000—5026

Figure 8. Histopathological alteration in the brain tissue of the control and treated groups. Hippocampus of regions stained with H&E (A) and Toluidine blue stain (B) (bar = 100 and 50 mm). Group I (6 weeks), the control group, showed a normal appearance and a high density of neurons with a light normal nuclear stain. Compared to the control group, Group II (6 weeks), which was made up of neurons that had dark nuclear staining (arrows) and focal disappearance, there was less density and necrosis in the D-gal group, which showed shrinking neurons with focal disappearance. In Group III (6 weeks), treatment with GNL revealed a protective effect, which showed mild neurodegeneration (thin arrows) with highly pronounced normal neurons (thick arrows). Normal appearance of neurons shown in Group IV (6 weeks) Treatment only. In Group V (4 months), a mild decrease in neuronal density has been shown compared to the control. However, severe neural loss, degeneration, and necrosis (arrows) were seen in old mice in Group VI (16 months) magnification 40X.