Research Paper Volume 16, Issue 7 pp 5987—6007
Identification and validation of the biomarkers related to ferroptosis in calcium oxalate nephrolithiasis
Figure 6. Fer-1 reduces CaOx-induced LPO and apoptosis, as well as LAMP2 and MDM4 expression in CaOx nephrolithiasis rat models. (A) TUNEL and DHE staining techniques are employed to evaluate apoptotic damage and the production of reactive oxygen species (ROS) in renal tissues (magnification×40; scale bar, 400 μm). (B) Representative immunofluorescence images show LAMP2 (green) and MDM4 (red) detection in renal tissues (magnification×40; scale bar, 400 μm).