Research Paper Volume 16, Issue 7 pp 6054—6067
Identification of a novel disulfideptosis-related gene signature for prognostic implication in lower-grade gliomas
Figure 5. Relationship between IQGAP1 and viability of U87 cells. (A) Immunohistochemistry of IQGAP1 protein expression in GBMLGG and counterpart normal adjacent tissues. (B) qRT-PCR assay shows the transcriptional levels of the IQGAP1 gene with GAPDH used as the loading control in U87 cells. (C) Effect of sh-IQGAP1 on the proliferation of U87 cells was detected by CCK-8 assays. (D) The cell cycle distribution of U87 cells were measured using flow cytometry analyses. (E) Apoptosis of U87 cells induced by cisplatin (20 μM) were measured by flow cytometry analyses. Data are presented as the mean ± SD for three independent experiments (*P < 0.05).