Online ISSN: 1945-4589

Aging-US: Exosomes attenuate ischemic retinal injury in vitro and in vivo

09-08-2021

Aging-US published a Special Collection on Eye Disease which included "Exosomes derived from BDNF-expressing 293T attenuate ischemic retinal injury in vitro and in vivo" which reported that this study investigated whether exosomes derived from BDNF-expressing 293T cells can be internalized by ischemic retinal cells and exert neuroprotective roles.

The results demonstrated that 293T-Exo significantly attenuated the loss of cell proliferation and cell death in R28 cells in response to oxygen-glucose deprivation treatment. Mechanistic studies revealed that the endocytosis of 293T-Exo by R28 cells displayed dose- and temperature-dependent patterns and may be mediated by the caveolar endocytic pathway via the integrin receptor.

Dr. Genlin Li and Dr. Yanling Wang said, "Retinal ischemia-reperfusion (I/R) emerges in many ocular diseases and is a leading cause of neuronal death and dysfunction, resulting in irreversible visual impairment or blindness."

"Retinal ischemia-reperfusion (I/R) emerges in many ocular diseases and is a leading cause of neuronal death and dysfunction, resulting in irreversible visual impairment or blindness."

Growing reports demonstrated that retinal ischemia is a primary contributor to the pathogenesis of multiple diseases, such as retinal vascular occlusions, diabetic retinopathy, central retinal vein occlusion, as well as age-associated macular degeneration. Exosomes are synthesized and released by various cell types and transport active biological molecules to regulate the physiological activities of recipient cells. Thus, exosomes play an essential role in intercellular communication. Therefore, understanding the mechanism of exosome uptake would promote the development of more efficient delivery systems for disease treatment.

Brain-derived neurotrophic factor, a member of the nerve growth factor gene family, is an essential multi-functional factor in various neuronal processes, including learning and memory, dendritic and synaptic plasticity, and axonal growth.

Figure 9. Endocytosis of 293T-Exo by retinal neurons and RGCs. Ischemia injury promoted endocytosis of 293T-Exo by retinal neurons and RGCs. The nuclei were stained with DAPI (blue), 293T-Exo was stained with fluorescent-tag (green), retinal neurons were stained with Beta III tubulin (red), and the nuclei of RGCs were stained with BRN3A (magenta). Scale bar = 25 μm.

The Li/Wang Research Team concluded in their Aging-US Research Output "the results suggest that 293T-Exo is endocytosed by retinal cells through the caveolar endocytic pathway via the integrin receptor. In addition, 293T-Exo exerts a neuroprotective role in the ischemic retina, both >in vitro and in vivo. The findings from the present study demonstrates a significant therapeutic potential of exosomes and provides an understanding of how to develop exosome-based therapies for retinal ischemia."

Full Text - https://www.aging-us.com/article/202245/text

Correspondence to: Genlin Li email: ligenlin2018@163.com and Yanling Wang email: wangyanling999@vip.sina.com

Keywords: retinal ischemia, exosome, brain-derived neurotrophic factor, endocytosis, apoptosis

About Aging-US:

Aging publishes research papers in all fields of aging research including but not limited, aging from yeast to mammals, cellular senescence, age-related diseases such as cancer and Alzheimer’s diseases and their prevention and treatment, anti-aging strategies and drug development and especially the role of signal transduction pathways such as mTOR in aging and potential approaches to modulate these signaling pathways to extend lifespan. The journal aims to promote treatment of age-related diseases by slowing down aging, validation of anti-aging drugs by treating age-related diseases, prevention of cancer by inhibiting aging. Cancer and COVID-19 are age-related diseases.

Aging is indexed by PubMed/Medline (abbreviated as “Aging (Albany NY)”), PubMed Central, Web of Science: Science Citation Index Expanded (abbreviated as “Aging‐US” and listed in the Cell Biology and Geriatrics & Gerontology categories), Scopus (abbreviated as “Aging” and listed in the Cell Biology and Aging categories), Biological Abstracts, BIOSIS Previews, EMBASE, META (Chan Zuckerberg Initiative) (2018-2022), and Dimensions (Digital Science).

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