Research Paper Volume 1, Issue 1 pp 68—80

Fat accumulation in Caenorhabditis elegans triggered by the electrophilic lipid peroxidation product 4-Hydroxynonenal (4-HNE)


Figure 1. Increased level of 4‑HNE-protein adducts in C. elegans with silenced gst‑10 gene. RNAi silencing of gst‑10 was carried out as previously described [21] in C. elegans strain Bristol-N2; worms fed bacteria transformed with an insert-free L4440 feeding vector served as the control. The level of 4‑HNE-protein adducts was determined in 5-day-old worms by competitive ELISA [93]. The values shown are means ± S.D. of 4 biological replicates (independently grown batches of worms) per group.