Research Paper Volume 1, Issue 1 pp 68—80

Fat accumulation in Caenorhabditis elegans triggered by the electrophilic lipid peroxidation product 4-Hydroxynonenal (4-HNE)


Figure 2. Lipid content of C. elegans with altered expression of gst‑10. (A) and (B): worms fed bacteria carrying the insert-free L4440 feeding vector (control) and worms subjected to RNAi against gst‑10. Lipid content was measured by Nile red fluorescence (A) and by Sudan black staining (B). (C): lipid content (by Sudan black) in worms transformed with the insert-free pPD49.26 vector (control) and in four transgenic C. elegans lines that overexpress gst‑10. Bars represent means ± S.D. (n = 50 worms per group) of pixel intensities integrated on worm micrographs.