Research Paper Volume 1, Issue 1 pp 68—80

Fat accumulation in Caenorhabditis elegans triggered by the electrophilic lipid peroxidation product 4-Hydroxynonenal (4-HNE)


Figure 3. Effects of RNAi against aldehyde dehydro-genases alh-1 and alh-10. (A) Effect of RNAi against alh-1 and alh-10 on NAD+-dependent 4‑HNE oxidation capacity of worm homogenates. (B) and (C): Fat content in C. elegans subjected to RNAi against alh-1 and alh-10, determined by Nile red (A) and Sudan black (B) staining followed by pixel integration. Means ± S.D. (n = 51 worms per group) are shown. In all experiments, statistical significance versus insert-free L4440 feeding vector control was assessed by the t‑test with Bonferroni correction.