Research Paper Volume 1, Issue 1 pp 68—80

Fat accumulation in Caenorhabditis elegans triggered by the electrophilic lipid peroxidation product 4-Hydroxynonenal (4-HNE)


Figure 6. Effect of increased 4‑HNE levels on malonyl-CoA concentration. C. elegans were either treated with 1 mM 4‑HNE according to the protocol described in the legend of Figure 5 (A) or subjected to RNAi to gst‑10 (B)). The results were normalized to the respective control and are shown as mean ± S.D. of 3 independently grown worm populations. In (A), the control (C. elegans Bristol-N2 in liquid culture in the presence of E. coli OP50 but without 4‑HNE) contained 16.5 ± 1.2 ng malonyl-CoA/mg dry weight; in (B), the level of malonyl-CoA in the control (C. elegans Bristol-N2 grown on plates seeded with E. coli HT115(DE3) transformed with insert-free L4440 feeding vector) was 12.2 ± 0.8 ng/mg dry weight.