Research Paper Volume 1, Issue 1 pp 131—145

Figure 5. Sch9p mediates TOR-dependent increases in mitochondrial function. Comparative analysisof mitochondria-related parameters in wild-type (wt), tor1Δ, sch9Δ and sch9Δtor1Δ strains in the DBY2006 genetic background. (A) Mitochondrial oxygen consumption. (B) Mitochondrial translation as described in Figure 1. (C) Western blot of the Cox1p, Cox4p, porin and actin OXPHOS components in the four strains using 100 μg of whole cell extract in each lane. We use the ratio of porin to actin as one measure of mitochondrial abundance per cell (which is virtually the same between the strains) and the ratio of Cox subunits to porin to demonstrate their specific increase per mitochondrial mass.