Research Paper Volume 1, Issue 2 pp 191—211

Figure 2. Expression pattern produced by GeneSwitch drivers and UAS-GFP reporter in larvae. The crosses are the same as Figure 1, but larvae were cultured in the presence and absence of drug in the food, from hatching to the indicated developmental stage. A. Expression patterns in 3^rd instar larvae and dissected tissues. For the Elav-GS driver ("Elav") a 1:10 dilution of drug was used because of the toxic effects of drug observed in larvae with this driver. Pictures were taken at the magnification of 25X, 100X, 20X, 50X, 100X, 80X, for whole larvae, brain, gut, salivary gland, imaginal discs, and fat body, respectively. B. Expression patterns in the three larval stages. For Elav-GS a 1:10 dilution of drug was used to avoid toxic effects. GFP pictures were taken at the magnification of 100X, 50X, 25X, for 1^st instar, 2^nd instar, and 3^rd instar, respectively. C. Expression in 3^rd instar larvae using Act-GS-255B and titrations of drug. ETOH indicates the ethanol solvent for the drug alone. Pictures were taken at the magnification of 25X.