Research Perspective Volume 1, Issue 7 pp 669—673

SIRT1 performs a balancing act on the tight-rope toward longevity


Figure 1. Loss of SIRT1 has minimal impact on gluconeogenesis in primary hepatocytes. (A) Glucose output from primary hepatocytes isolated from control and SIRT1 LKO mice. Cells were treated with DMSO (white bars) or 10 μM forskolin (black bars) and incubated for 6 h in glucose free DMEM supplemented with 20 mM sodium lactate and 2 mM sodium pyruvate. Glucose output was measured in culture medium using a glucose oxidase kit (Sigma). Data represent mean + SD. (B-C) SIRT1 deficiency in primary hepatocytes reduces the induction of PGC-1α (B) but not PEPCK (C) message in response to 10μM forskolin treatment. mRNA from primary hepatocytes treated with DMSO (white bars) or forskolin (black bars) were analyzed using qPCR. Data represent mean + SD.