Research Paper Volume 2, Issue 7 pp 393—414
Chemical genetic screen identifies lithocholic acid as an anti-aging compound that extends yeast chronological life span in a TOR-independent manner, by modulating housekeeping longevity assurance processes
Figure 2. The pex5Δ mutation alters the abundance of many proteins recovered in total cell lysates, purified ER and mitochondria of CR yeast. (A) The spectra of proteins recovered in total cell lysates, purified ER and mitochondria of WT and pex5Δ cells that were grown under CR on 0.2% glucose and taken for analyses at the indicated time-points. (B) Functional categories of proteins that were enriched or depleted in the total cell lysate, ER and mitochondria of pex5Δ cells (as compared to WT cells) under CR conditions. Lack of 91 of these proteins increased the CLS of yeast under CR, suggesting their essential pro-aging role in longevity regulation when calorie supply is limited.