Figure 2. MEF immortalization is characterized by a p53-dependent events. DNA content profile per cell at p1/p5 (A), p5/p6 (B) and p6/p10 (C). (D) Fold change of miR-34a and p53 transcripts at various passages normalized to that of MEF at passage 0. (E) p53 protein level at the various passages. The value reported under each lane represents the average of two independent experiments.