Research Paper Volume 4, Issue 12 pp 923—931

P73 and age-related diseases: is there any link with Parkinson Disease?


Figure 3. Tyrosine hydroxylase levels correlates with p73 transactivation potential. N2a cells were transiently transfected with human TAp73β or shRNAs specific for exon 13; 48 hours later, cells were collected and processed. Semi-quantitative PCR showed that knock-down of exon 13 lead to a shift from α to β (A). Real-time PCR shows an increase of about 5 times in Th levels (B). In order to test efficiency of transfection, p73 levels were monitored (C). Experiment has been reproduced at least 3 times (data are represented as mean +/− SD). KD = knock-down, GAPDH = Glyceraldehyde 3-phosphate dehydro-genase, untr. = untreated. Experiment has been reproduced at least 3 times.