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Research Paper Volume 6, Issue 5 pp 369—379

Long term exposure to L-arginine accelerates endothelial cell senescence through arginase-II and S6K1 signaling

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Figure 6. Arg-II and S6K1 mediate the chronic effect of L-arginine on cell senescence. Endothelial cells were treated as in Fig. 5. (A) SA-β gal assay. (B) Western blot analysis of eNOS, ICAM-1, VCAM-1. Tubulin served as loading control. (C) DHE staining for detection of O2.−, DAF-2DA staining for detection of NO. The graphics at the corresponding right panels present the quantification of the signals. *p < 0.05 vs rAd/U6-LacZshRNA control+0.5 mmol/L L-arginine. Scale bar = 200 μm