Figure 6. RNAi inhibition of both pch-2 in sir-2.1 over-expressing worms, and sir-2.1 in pch-2 over-expressing worms, shortens lifespan. (A) Purple curves show lifespans of sir-2.1 over-expressing worms, while red curves show lifespans of pch-2 over-expressing worms. In sir-2 over-expressing worms, pch-2 RNAi significantly decreased lifespan (56% and 75% decrease in median and maximum lifespan, respectively). In pch-2 over-expressing worms, sir-2.1 RNAi induced a 57% and 70% decrease in median and maximum lifespans (p <0.0001 for both effects). (B) qRT-PCR validated the RNAi effect, indicating a significant reduction (~90%) of pch-2 transcipts in sir-2 over-expressing worms that were treated with pch-2 RNAi. (C) qRT-PCR validating a significant reduction (~70%) of sir-2 transcripts in pch-2 over-expressing worms that were treated with sir-2 RNAi. *p <0.001.