Research Paper Volume 7, Issue 9 pp 701—712

The loss of LRPPRC function induces the mitochondrial unfolded protein response


Figure 2. Upon LRPPRC siRNA-induced UPRmt, mitochondrial proteostasis is restored. UPRmt in SH-SY5Y cells treated with control or LRPPRC siRNA for 2, 3, 4 or 5 days and transferred for 24 hours in low glucose medium. (A) Total protein extracts were analyzed by Western using anti-LRPPRC, anti-COX I, anti-COX IV and anti-β-Actin antibodies. COX IV/COX I ratios are indicated. (B) The same protein extracts as in panel A were also analyzed by Western using anti-LRPPRC, anti-HSP70, anti-HSP60, anti-ClpP and anti-β-Actin antibodies. Ratios relative to the control siRNA are indicated. (For all panels, quantifications are based on data from three independent experiments; average values are shown and error bars indicate s.d.; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 by one way ANOVA for panel A and one sample t-test for panel B).