Research Paper Volume 8, Issue 3 pp 427—439

Expression of amyloid-β in mouse cochlear hair cells causes an early-onset auditory defect in high-frequency sound perception


Figure 2. Aβ42Arc is expressed along the hair cells in the cochlea. Hematoxylin and eosin staining of sectioned cochleae from 4-month-old non-Tg (A) and Tg(Math1E-Aβ42Arc)1Lt mice (B). OHC, outer hair cell (arrows); tm, tectorial membrane; bm, basilar membrane. (C-E) Immunostaining of cochlear sections at age 4 months using Aβ antibody (6E10). (C) Antibody 6E10 did not stain the cochlea of non-Tg mice. (D) The cochlear OHCs from Tg(Math1E-Aβ42Arc)1Lt were strongly stained by 6E10 (arrows). (E) Possible aggregation of an Aβ-like protein (arrows) in the cochleae of Tg(Math1E-Aβ42Arc)1Lt mice. (F-H) Immunoelectron microscopy of hair cells in Tg(Math1E-Aβ42Arc)1Lt cochlea at age 4 months. An Aβ-like protein accumulated in the endoplasmic reticulum (F), stereo cilia (G), and plasma membrane (H). Arrowheads: clusters of Aβ-like protein were observed around the stereo cilia (G) and plasma membrane (H). IHC: inner hair cell. Arrows: Aβ-like protein were also observed within the neighboring cells (H).