Figure 7. Mineralocorticoid receptor (MCR) expression and distribution in the cochlea of the young adult (3 mon) CBA/CaJ mouse. (a) Multiplex RT-PCR was performed as described in the text. This experiment includes the absence of reverse transcription (control lane -RT). The mRNA expression of MCRs in tissue from heart (H), muscle (M) and spiral ganglion neurons (SG), where the product of RT-PCR is about 245 bp. -RT- reverse transcription without primers (as the negative control). L-Ladder: DNA molecular weight markers. (b) The protein expression of MCRs in SG was detected using immunohistochemistry staining (brown color, MCR+). A portion of the limbus is shown as a cochlear region not displaying significant MCR staining (MCR-).