Research Paper Volume 8, Issue 11 pp 2777—2789
Long-term caloric restriction in ApoE-deficient mice results in neuroprotection via Fgf21-induced AMPK/mTOR pathway
Figure 6. (A-F) Representative immunohistochemical images (original magnification x400) of PSD95 protein expression in brain of short-term (A and B), mid-term (C and D) and long-term (E and F) ad libitum- (AL, left panels) and caloric-restricted-fed (CR, right panels) ApoE-/- mice. Mice were fed either AL or CR (60% of ad libitum) for a short-term (4 weeks; n=14), mid-term (20 weeks; n=14) or long-term (64 weeks; n=14). Note the decrease of PSD95-positive neurons in the AL-fed ApoE-/- mice while a long-term CR delayed the decline of PSD95-positive neurons in the ApoE-/- mice (indicated by arrows).