Figure 5. Autophagy depressed germ cell cyst breakdown and increased the number of surviving gem cells after 3 days of treatment. (A) IF staining for MVH (green) of control, rapamycin and 3-MA treated mouse ovaries for 3 days. (B) Percentage of germ cells in cysts and follicles in the three groups after 3 days treatment. (C) Average number of survived oocytes in the three groups. (D) Level of BECLIN1 protein in control, rapamycin and 3-MA treated ovaries (6 h). The results are presented as mean ± SD. *P < 0.05; **P < 0.01.