Figure 1. The hiPSC seeding density is a determining factor in differentiating the two forms of cardiomyocyte clusters. (A and B) The beating rates of two forms of hiPSC-CMs; left panel: sheet-shaped CMs; right panel: net-shaped CMs. The movies are shown in the supplemental movies. (C) Time course of pluripotent growth and subsequent cardiac differentiation showing the medium and small molecules used on each day of differentiation. (D) Representative images of hiPSCs seeded at different densities on E8 and subsequently differentiated.