Research Paper Volume 10, Issue 4 pp 532—548

A net-shaped multicellular formation facilitates the maturation of hPSC-derived cardiomyocytes through mechanical and electrophysiological stimuli


Figure 3. The net-shaped CMs act more mature at the transcriptional level than the sheet-shaped CMs. (A) NOISeq method of screening differentially expressed genes between net-shaped CMs and sheet-shaped CMs; some highly up-regulated genes are shown in a heat map. (B) Real-time quantitative polymerase chain reaction analyses of cardiac structure-related genes (TNNT2, LRCC39, MYH6, MYH7, MYL2, and MYL7) and cardiac function-related genes (SEARA2A, RYR2, SCN5A, CACNA1C, KCNH2, KCNQ1, and KCNJ2) to verify the mRNA levels in the sheet-shaped and net-shaped CMs. * Statistically significant differences between individual groups (n≥3; *P<0.05, **P<0.01, ***P<0.001).