Research Paper Volume 10, Issue 4 pp 532—548

A net-shaped multicellular formation facilitates the maturation of hPSC-derived cardiomyocytes through mechanical and electrophysiological stimuli

Figure 4. The motion analysis reveals significant physical differences among CMs with different shapes. (A and B) The MHMA of sheet-shaped CMs and net-shaped CMs; left panel: sheet-shaped CMs; right panel: net-shaped CMs. (C and D) The MLMA of sheet-shaped and net-shaped CMs; left panel: sheet-shaped CMs; right panel: net-shaped CMs. (E) The direction and velocity of two forms of hiPSC-CMs. Movement to the right is shown in red and to the left is shown in blue; deeper colors represent a greater velocity. (F) Movement cycles of the two forms of hiPSC-CMs. (G and H) The MHV of sheet-shaped and net-shaped CMs. (I and J) The MLV of sheet-shaped and net-shaped CMs; left panel: sheet-shaped CMs; right panel: net-shaped CMs. All data are expressed as means±S.E.M. * Statistically significant differences between individual groups (n≥3; *P<0.05, **P<0.01, ***P<0.001).