Figure 6. Robust beating of net-shaped CMs enhances connexin expression. (A and D) Immunostaining showing increased levels of connexin-43 (green) in net-shaped CMs. Troponin (red) and DAPI (blue) staining are also shown. (C) Quantitative PCR analysis of N-CAD, JUP, and CX43 expression. (B and E) Up-regulation of the CX43 protein in net-shaped CMs. All data are expressed as means±S.E.M. (n≥3; ***P<0.001 using Student’s t-test).