Research Paper Volume 10, Issue 4 pp 549—560

Unrepaired DNA damage in macrophages causes elevation of particulate matter- induced airway inflammatory response

Figure 4. Genetic deletion of RAD50 in macrophages increases cytokine production following particulate matter (PM) stimulation. BMDMs from mice with RAD50 deletion in myeloid cells (RAD50flox/flox-LysMcre) and wildtype (WT) mice (RAD50flox/flox) were treated with PM (100 μg·mL-1). (A) BMDMs were stimulated with PM for 3 hours. Representative images of alkaline comets. (B) Quantification of DNA damage. (C) BMDMs were stimulated with PM for 24 hours. Relative levels of Cxcl1, Cxcl2 and Ifn-γ were determined using quantitative PCR. Data are presented as means ± SEMs across at least 3 independent experiments. *p < 0.05; **p < 0.01.