Research Paper Volume 10, Issue 4 pp 549—560

Unrepaired DNA damage in macrophages causes elevation of particulate matter- induced airway inflammatory response

Figure 6. RAD50flox/flox-LysMcre mice display exacerbated airway inflammation in response to particulate matter (PM) exposure. RAD50flox/flox-LysMcre male mice and their wildtype littermates (n =5, 6, or 7 per group) were instilled intratracheally with PM at 100 μg·d-1 or the equivalent volume of normal saline (NS) as control for 2 days, and after 24 hours. In the bronchoalveolar lavage fluid, (A) the total number of inflammatory cells was quantified and the number of neutrophils was calculated. (B) Expressions of Il6, Cxcl1, Ifn-γ and Il17 levels in the lung tissue were determined using real-time PCR and ELISA. (C) Representative images of lung sections stained with hematoxylin and eosin (H & E). (D) Semiquantified inflammation score of the H & E staining (n =10 images per group). *p < 0.05, **p < 0.01,***p < 0.001.