Figure 4. Decreased adiposity and increased glucose tolerance in aging Pik3c3 mutant mice. Total body weight and gWAT and gWAT: body weight ratio of male (24 MO) fl/fl and mutant mice (n=4 for each group) were plotted (A). Blood glucose levels in GTT were plotted (B) and area under the curve was plotted in (C). Insulin resistance was calculated by homeostatic model assessment of (HOMA) using fasting glucose and fasting insulin levels and plotted in D. The significance levels were analyzed by unpaired Student’s t-test using means and SDs and designated as *p<0.05, **p<0.01, or ***p<0.001 and ns=not significant (p>0.05).