Figure 5. PIK3c3 mutation reduced adipogenesis and enhanced browning in the gWAT of old mice Expression of adipogenic factors (aP2, Cebp-α and Ppar-γ) were evaluated in 24 MO fl/fl and Pik3c3 mutant male mice (n=4 for each group). Both mRNA (A) and protein expression (B) were plotted. Relative intensity of protein bands were normalized with the corresponding α-tubulin bands and plotted (C). Expressions of Ucp1 and Pgc1a mRNA (D) protein expressions were presented in (E). Relative intensities of protein expression were presented in (F). The significance levels were analyzed by unpaired Student’s t-test using means and SDs and designated as *p<0.05, **p<0.01, or ***p<0.001 and ns=not significant (p>0.05).