Figure 6. Granulosa cells from POF3 suffer apparent mitochondrial apoptosis and ER stress. (A-D) WB and grey analysis of BCL2, GRP78 and BAX expression in the granulosa layer from POFs (POF1 to POF4). (E-I) qRT-PCR analysis of Bok, Bak1, Grp78, Grp94 and Atf4 mRNA abundance in the granulosa layer from POFs (POF1 to POF4). GL represents the granulosa layer. Values are means ± SEM of three experiments. Asterisks indicate significant differences (* P<0.05 and ** P<0.01).