Research Paper Volume 10, Issue 6 pp 1281—1293

CircSNCA downregulation by pramipexole treatment mediates cell apoptosis and autophagy in Parkinson’s disease by targeting miR-7

Figure 5. CircSNCA increased SNCA expression by downregulating miR-7 and influenced the expression of apoptosis-related genes. (A) Endogenous competition between circSNCA and SNCA mRNA for miR-7 binding. (B) The relative expression of circSNCA detected by qRT-PCR increased after overexpression (circSNCA-OE) in the MPP+ group. *P < 0.05 compared with NC. (C) The relative expression of circSNCA detected by qRT-PCR decreased after circSNCA knockdown in the MPP+ group. *P < 0.05 compared with NC. (D) The relative expression of miR-7 detected by qRT-PCR decreased after circSNCA overexpression in the MPP+ group. *P < 0.05 compared with NC. (E) The relative expression of miR-7 detected by qRT-PCR increased after circSNCA knockdown in the MPP+ group. *P < 0.05 compared with NC. (F) The protein expression of SNCA and pro-apoptotic genes (CASP3, BAX, PTEN and P53) detected by western blot increased in the circSNCA overexpression group, decreased in the circSNCA knockdown group, while that of the anti-apoptotic gene (BCL2) showed the opposite tendency. *P < 0.05 compared with NC group, #P < 0.05 compared with circSNCA-OE.