Figure 1. qRT-PCR analysis of nicotinic acetylcholine receptor (nAChRs) subunit mRNA in fetal ovaries cultured for 4 days in the presence of 1mM or 10mM nicotine. The expression levels were normalized to that of Gapdh gene as a control. All experiments were repeated at least three times. Results are presented as mean ± SD in comparison to control. (*) and (**) indicate significant (P < 0.05) and highly significant (P < 0.01) difference, respectively.