Figure 4. Detection of ROS production in MII oocytes during aging in vivo. (A) Representative confocal images of DCF fluorescence in control and resveratrol-treated oocytes. ROS production was measured by fluorescent probe DCFA-DA (green). Scale bar: 100 μm. (B) The relative levels of intracellular ROS determined by quantitative fluorescence intensity. Data are expressed as mean ± SEM of at least 5 independent experiments. *Significantly different (P < 0.05).