Research Paper Volume 10, Issue 7 pp 1597—1608

Aging exacerbates mortality of Acinetobacter baumannii pneumonia and reduces the efficacies of antibiotics and vaccine

Figure 3. Enhanced levels of cytokines after A. baumannii infection in aged mice. Young and aged mice were infected intratracheally with 5×106 CFU of LAC-4. At 0 h and 24 hpi, serum and BALF were collected and mRNA was isolated from lungs. TNFα, IL-6, and IL-1β levels in serum (A) and BALF (B) were detected by ELISA. (C) TNFα, IL-6, and IL-1β mRNA expression in lungs were detected by real-time PCR. Data are presented as mean ± SEM of five mice per condition and represent one of 2 independent experiments. Statistical analyses were performed by Student’s t test. *, P < 0.05, **, P < 0.01.